ECTS
ECTS Course Catalogue
Course details
Course code: BBS20381o13Semester: 2013/2014 summer
Name: Techniques of cell and tissue cultures
Major: Biology
Study Type: second cycle
Course type: compulsory
Study Semester: 2
ECTS points: 4
Hours (Lectures / Tutorials / Other): 15 / 15 / 0
Lecturer: dr hab. Wojciech Kruszyński, dr Magdalena Moska
Language of instruction: Polish
Learning outcomes: Knowledge: Student: W1 – has the knowledge allowing to interpret the natural phenomena and processes in research work and practical activities [KB2_W01]; W2 - knows the research methodology obligatory in natural sciences [KB2_W01]; W3 - formulates research hypotheses and solves basic scientific problems[KB2_W01]; W4 - knows how to create an interesting multimedia presentation [KB2_W01]. W5 – knows methods of chemical and instrument al analysis, with special attention paid to physico-chemical basis [KB2_W01_BT]; W6 - has the knowledge of research methods and techniques used in laboratories [KB2_W01_BT]; W7 - knows the rules of biological material handling [KB2_W01_BT]; W8 - has the knowledge essential for laboratory equipment operating [KB2_W01_BT]; W9 - demonstrates advantages and disadvantages of particular analytical techniques [KB2_W01_BT]. W10 – characterizes techniques, methods and application of cell and tissue cultures of plant and animal organisms [KB2_W05_BT]; W11 - describes the rules of cytogenetic preparations making, their staining and proper analysis [KB2_W05_BT]. Skills: Student: U1 – effectively and safely uses research apparatus and measurement devices used in biology [KB2_U02]; U2 - is able to select suitable measurement devices and measure needed values of physiological and morphological features [KB2_U02]. U3 – uses the methods applied in laboratory analytics: optical, spectroscopic, electroanalytical, separation ones [KB2_U01_BT]; U4 - is able to assess factors influencing the result of laboratory analysis avoiding pre- and post-analytical mistakes [KB2_U01_BT]; U5 - interprets the results of laboratory analysis based on standards and reference values [KB2_U01_BT]; U6 - collects and interprets the data form analysis and formulates conclusions based on them [KB2_U01_BT]. U7 – operates various types of microscopes [KB2_U07_BT]; U8 - is able to make the preparations using light and electron microscopy [KB2_U07_BT]; U9 - performs histological staining of the preparations [KB2_U07_BT]; U10 - is able to use an application of SEM-EDS microscopy in determination of elemental composition of the examined substance [KB2_U07_BT].
Competences: Personal and social competences: Student: K1 – demonstrates creativity and is able to determine priorities in task realization taking into account team work [KB2_K05]. K2 – obeys the rules of occupational health and safety in biological laboratories, demonstrating responsibility for an assessment of dangers resulting from research techniques used [KB2_K07]; K3 - demonstrates the care of creation of safe work conditions [KB2_K07].
Prerequisites: histology, genetics, biochemistry, physiology
Course content: material used in cytogenetics and kind of tissue cultures, plant cell and tissue cultures, animal cell and tissue cultures, usefulness of cell and tissue culture in biology, medicine and veterinary medicine, preparation and banding chromosome slides (conventional stain and banding stain, FISH), karyograms and their analysis, cytogenetic diagnostic of animals and human, techniques of banding of germ cells.
Recommended literature: 1. Stokłosowa S. (red.). 2006. Hodowla komórek i tkanek. PWN. Warszawa 2. Rogalska S., Małuszyńska J., Olszewska M.J. 2005. Podstawy cytogenetyki roślin. PWN. Warszawa 3. Świtoński M., Słota E., Jaszczak K. 2006. Diagnostyka cytogenetyczna zwierząt domowych. Wydawnictwo Akademii Rolniczej im. A. Cieszkowskiego w Poznaniu.
Assessment methods: Completion of the course: combined passing of tutorials and lectures with a mark, based on mean mark obtained from two written tests and oral statements during the classes. Written tests (two, 20-30 minutes on the 5th and 10th classes) including the issues discussed on tutorials and lectures. Presence on tutorials is obligatory; student is allowed to have only one unjustified absence. In case of unjustified absence the student is obliged to score a suitable part of material.
Comment: specialization: Laboratory Techniques in Biology