Courses list

Wersja polska

Course code: NBSS10147o16
Semester: 2016/2017 winter
Name: Basic of biotransformations
Major: Biotechnology
Study Type: first cycle
Course type: compulsory
Study Semester: 5
ECTS points: 5
Hours (Lectures / Tutorials / Other): 15 / 45 / 0
Lecturer: Prof. dr hab. Ewa Huszcza
Language of instruction: Polish / English


Learning outcomes: Knowledge Students after completion of the course explain reasons for the widespread use of enzymatic transformations in practical applications; characterize major processes which utilize biotransformations; explain mechanisms of the reactions most often used in biotransformations; identify products of enzymatic reactions; indicate type of biocatalyst for a selected transformation. Skills: Students perform on their own microbial transformations, prepare biocatalysts, conduct isolation of the products, determine parameters of the process, control transformation progress, operate analytical equipment, interpret and analyze the results.

Competences: Students are able to work in a team, and understand the role of following the rules indicated in the instructions, due to exposure to chemicals and microorganisms.

Prerequisites: Organic chemistry, biochemistry, microbiology

Course content: Catalytic properties of enzymes. Hydrolysis reactions. Enzymatic reduction of carbonyl group. Hydroxylation reactions catalyzed by P450 cytochrome monooxygenases; preparation of hydroxyl derivatives with the use of dehydrogenases. Use of biotransformations in steroid chemistry. Enzyme-catalyzed isomerization reactions. Use of biotransformations in food industry.

Recommended literature: 1. Kołek T.: Biotransformacje, Wydawnictwo Akademii Rolniczej, Wrocław, 2005; 2. Kołek T., Bartmańska A.: Podstawy biotransformacji, Wydawnictwo Akademii Rolniczej, Wrocław, 2005; 3. Faber K.: Biotransformations in organic chemistry, Springer-Verlang, Berlin, Heidenberg 2000; 4. Holland H. L.: Organic synthesis with oxidative enzymes, VCH Publishers New York, 1992; 5. Kieslich K.: Biotechnology Vol. 6a, editor Verlag. Chemie, Weinhei 1984.

Assessment methods: Completion of the practicals, written reports from the practicals, colloquia, final project, written exam.

Comment: The course in English will be avaliable if minimum of 6 students sign up for a group. If less than 6 students will sign up for the group the course will be avaliable in Polish with the possibility of individual support in English.