ECTS
ECTS Course Catalogue
Course details
Course code: NBSS20195o16Semester: 2016/2017 winter
Name: Tissue Cultures
Major: Biotechnology
Study Type: second cycle
Course type: compulsory
Study Semester: 2
ECTS points: 4
Hours (Lectures / Tutorials / Other): 30 / 15 / 0
Lecturer: dr inż. Magdalena Wróbel-Kwiatkowska
Language of instruction: Polish / English
Learning outcomes: The student knows the contemporary goals and techniques of obtaining and maintaining the cells alive in culture in vitro, both of plant or animal cells.
Competences: Establishment and maintenance of the surviving cells in tissue culture. It is able to resolve the processes related to proliferation, differentiation and death of aging cells in vitro.
Prerequisites: None
Course content: Cytology, the organization of DNA, cell cycle and its regulation. Methods for in vitro culture, meristematic culture, callus culture, somatic embryogenesis, organogenesis. Micropropagation of plants. Preparation and use of hybrids and their significance. Release of plants from viruses, the use of somatic embryos in the creation of artificial seeds. Cell suspensions and their use in bioreactors. Obtaining haploid plants and doubled haploid lines. Transformation in plants, gene isolation, gene structure, binary vector system, the introduction of the gene structure of plant cell-method: Vector, direct. Regeneration, and identification of transgenic plants. Resistance to stress, resistance to herbicides, pathogens, pests, modified traits-the value of food, industrial uses, in medicine. Stem cells in various stages of development, their sources, biology and application in biology and medicine. Breeding of gametes and embryos. In vitro fertilization. Breeding of embryos to the blastocyst stage. The use of embryonic stem cells in biology and medicine. Cultures of cells and tissues as a source of transplants. Adaptive cell transfer. Auto-grafts, allo-and xenogeneic. Transplantation immunology. Established in vitro tumor cell lines. The differences between normal and tumor cell. Methods of isolation, preparation and cultivation of tumor cells. Methods used in studies using tumor cell lines. Tests proliferation in vivo. Testing adhesion and migration. Evaluation of metastatic potential. Use of tumor lines in studies of new anticancer therapy.
Recommended literature: 1. Alberts B. et al: Fundamentals of cell biology. Introduction to molecular biology. Ed. Science. OWN 1999, 2. Kilarski W.: Structural basis of cell biology. Ed. Science. OWN, Warsaw 2007, 3. Malepszy S. Plant Biotechnology ed. Science OWN, Warsaw 2009, 4. Michalik B. Application of biotechnological methods in plant breeding. Ed. DRUKPOL s.c., Cracow, 1996, 5. Michelin B.. Plant breeding with the elements of genetics and biotechnology. PWRiL, Poznań 2009, 6. Rogalska S., Maluszyńska J., Olszewska M. Fundamentals of plant cytogenetics. Ed. Science OWN, Warsaw 2005, 7. Brome S: Cell culture and tissues. Ed. Science. OWN, Warszawa 2004, 8. A janitor, arrived K. Plant cells under stress conditions. Volume II. Ed cells in vitro. Science. UAM, Poznań 2004, 9. Zwierzchowski L., et al: Animal Biotechnology. Ed. Science OWN, Warsaw 1997.
Assessment methods: After obtaining the signature of the individual classes, written exam test.
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