Courses list

Wersja polska

Course code: BŻS10072f16
Semester: 2016/2017 summer
Name: Tissue Engineering with Stem Cells
Major: Food Safety
Study Type: first cycle
Course type: optional
Study Semester: 4
ECTS points: 2
Hours (Lectures / Tutorials / Other): 15 / 15 / 0
Lecturer: dr Krzysztof Marycz
Language of instruction: Polish / English
The course taught in English if the group has ≥6 students. The course taught in Polish with a possibility of support in English if the group has <6 students.

Learning outcomes: Knowledge: knows the principles of aseptic work; knows the classification of cell and tissue cultures; knows methods of mesenchymal stem cells isolation and identyfication; has knowledge how to design biomaterials; is aware how to use techniques of cell and tissue culture in regenerative medicine. Skills: Student is able to conduct cell passage and maintains cell culture; is able to freeze cells and to defrost cells’ pellets; is able to determine cells’ morphology and cultures’ condition using inverted light microscope; is able to plan an experiment in order to determine biomaterials’ biocompatibility; is able to propose the way of biomaterials’ modification for different branches of regenerative medicine.

Competences: Student is responsible for his own cells’ cultures; is responsible for tasks, that are realized in groups during the tutorial; is conscious of importance of working in compliance with safety and health rules and keeps rudiments of aseptic working.

Prerequisites: Histology and cell biology

Course content: Cell culture. Tissue culture. Synthesis of alginate materials. Design of fibrin implants. Suspension of stem cells in fibrin. Suspension of stem cells onto modified metallic biomaterials. Aseptic working in a laminar flow hood. Centrifuge operation. Inverted microscope operation. Cytotoxicity assays (XTT or MTT).

Recommended literature: 1. Stokłosowa S. : Hodowla komórek i tkanek, PWN, Warszawa 2011; 2. Kawiak J. : Seminaria z cytofizjologii dla studentów medycyny weterynarii i biologii, Urban & Partner, Warszawa 2009; 3. Alberts B.: Podstawy biologii komórki T1.-T2. PWN, Warszawa 2009; 4. Mather J.P. Introduction to Cell and Tissue Culture.Theory and Technique, Springer 1998.

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