ECTS
ECTS Course Catalogue

Course details
Course code: BBS20180o10
Semester: 2010/2011 summer
Name: Techniques of cell and tissue cultures
Major: Biology
Study Type: second cycle
Course type: compulsory
Study Semester: 2
ECTS points: 5
Hours (Lectures / Tutorials / Other): 15 / 30 / 0
Lecturer: dr hab. Wojciech Kruszyński, dr Magdalena Moska
Language of instruction: Polish


Learning outcomes: During the course students acquire a knowledge of techniques, methods and using of plants and animals cell and tissue cultures and practical abilities in basic range enabling conduct cultivating cellular. Particularly, ability of set-up acquires and conducts of short-term cellular cultivating (selection proper utensil and growth media), preparation of chromosome preparations, chromosome banding and analysis of karyotypes.

Competences: Passing the course permits working in diagnostic laboratory and lead cytogenetic research.

Prerequisites: histology, genetics, biochemistry, physiology

Course content: Material used in cytogenetics and kind of tissue cultures, plant cell and tissue cultures, animal cell and tissue cultures, usefulness of cell and tissue culture in biology, medicine and veterinary medicine, preparation and banding chromosome slides (conventional stain and banding stain, FISH), karyograms and their analysis, cytogenetic diagnostic of animals and human, techniques of banding of germ cells.

Recommended literature: Stokłosowa S. (red.). 2006. Hodowla komórek i tkanek. PWN. Warszawa Rogalska S., Małuszyńska J., Olszewska M.J. 2005. Podstawy cytogenetyki roślin. PWN. Warszawa Świtoński M., Słota E., Jaszczak K. 2006. Diagnostyka cytogenetyczna zwierząt domowych. Wydawnictwo Akademii Rolniczej im. A. Cieszkowskiego w Poznaniu.

Assessment methods: Completion of laboratory training, written exam; minimum 60% of knowledge to pass.

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