ECTS
ECTS Course Catalogue
Course details
Course code: NBSS10149o10Semester: 2010/2011 summer
Name: Enzymology
Major: Biotechnology
Study Type: first cycle
Course type: compulsory
Study Semester: 6
ECTS points: 3
Hours (Lectures / Tutorials / Other): 15 / 30 / 0
Lecturer: prof. dr hab. Danuta Witkowska
Language of instruction: Polish
Learning outcomes: Students know mechanism and kinetics of enzyme activity, enzymes preparation production and purification with selective precipitation, gel filtration, specific adsorption, ion exchange, affinity chromatography.
Competences: Students are able to measure kinetics of enzymes action, know how to isolate enzymes from biological materials and are capable to produce technical and pure enzyme preparations.
Prerequisites: Organic Chemistry, Biochemistry, Microbiology
Course content: Mechanism and kinetics of enzyme action; catalytic and physicochemical properties, principle of enzyme isolation from animal, plant and microorganism sources (enzymes extraction, fractional precipitation, gel filtration, specific adsorption, ion exchange and affinity chromatography) and production of technical and pure enzyme preparations.
Recommended literature: 1. The enzyme catalysis process, Cooper A., Houben J., L., Chien L., C., New York, London 1988; 2. Microbial enzymes and biotechnology, Fogarty W., M., NewYork, London 1983; 3. Enzymes in food processing, Ed. Nagodawithane T., W., Reed G., Acad. Press INC 1993; 4. Biotechnology, Vol. 9, Enzymes, biomass, food and feed, Rehm H., J., Reed G., Ed. Reed G., Nagodawithane T., W., VCH Weinheim, New York 1997.
Assessment methods: Passing grades of laboratory course and written exam, minimum of knowledge to passing the course - 60%.
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