ECTS
ECTS Course Catalogue
Course details
Course code: BBS20371o14Semester: 2014/2015 winter
Name: Microscopic Techniques
Major: Biology
Study Type: second cycle
Course type: compulsory
Study Semester: 1
ECTS points: 4
Hours (Lectures / Tutorials / Other): 15 / 15 / 0
Lecturer: dr Krzysztof Marycz
Language of instruction: Polish / English
The course taught in English if the group has ≥6 students. The course taught in Polish with a possibility of support in English if the group has <6 students.
Learning outcomes: Knowledge: student can identify and characterize animal tissues; student can recognize and differentiate animal tissues on histological preparations; student can choose tools needed for visualization of particular biological structures. Skills: student can use light microscope and make observations of histological preparations; student can choose and decide about the most suitable research methods needs for resolving particular research problem.
Competences: Personal and social competences (attitudes and behaviors): student plans tasks and chooses methods for animal tissue isolation; student shows ethical attitudes for experimental animals; student understands the importance of the BHP rules compliance due to contact with animal-derived material and harmful factors in laboratory, shows responsibility for entrusted preparations and microscopy.
Prerequisites: histology, cell biology, biophysisc
Course content: Transmission and scanning electron microscopy. Types of light microscopes. Types of microscopy preparations. Fixation and staining techniques. Autoradiography. Types of cyto- and histochemical reactions. In situ hybridization. Fluorescence microscopy. Hardware and software picture analysis tools.
Recommended literature: 1. Pluta M.: Mikroskopia Optyczna. PWN, Warszawa, 1982; 2. Barbacki A.: Mikroskopia Elektronowa. Wydawnictwo Politechniki Poznańskiej, Poznań, 2007. 3. Burry R.W.: Immunocytochemistry – A practical guide for biomedical research. Springer, Cambridge, 2010. 4. Bozzola J.J., Dee Russell L.: Electron microscopy: principles and techniques for biologists. Jones&Bartlett Learning, 1999.
Assessment methods: Tutorial credit: checking the skills for making micrographs using light and electron microscopy, evaluation of mastery in work with microscopes, written examinations on grade, obligatory presence, one absence without excuse in case witch student must credit proper part of material, tutorial credit on the basis of grades’ average. Course credit: students are obligated to write test from lecture material. Final grade is based on lecture and tutorial grades average.
Comment: specialization: Laboratory Techniques in Biology