Courses list

Wersja polska

Course code: BŻS10072f14
Semester: 2014/2015 summer
Name: Tissue Engineering with Stem Cells
Major: Food Safety
Study Type: first cycle
Course type: optional
Study Semester: 4
ECTS points: 2
Hours (Lectures / Tutorials / Other): 15 / 15 / 0
Lecturer: dr Krzysztof Marycz
Language of instruction: Polish
The course taught in English if the group has ≥6 students. The course taught in Polish with a possibility of support in English if the group has <6 students.

Learning outcomes: Knowledge: W1 – knows the principles of aseptic work [BZ_W21]; W2 – knows the classification of cell and tissue cultures [BZ_W09; BZ_W10, BZ_W12]; W3 – knows methods of mesenchymal stem cells isolation and identyfication [BZ_W12]; W4 – has knowledge how to design biomaterials [BZ_W12]; W5 – is aware how to use techniques of cell and tissue culture in regenerative medicine [BZ_W10; BZ_W12] Skills: U1– is able to conduct cell passage and maintains cell culture [BZ_U01]; U2 – is able to freeze cells and to defrost cells’ pellets [BZ_U01]; U3 – is able to determine cells’ morphology and cultures’ condition using inverted light microscope [BZ_U01]; U4 – is able to plan an experiment in order to determine biomaterials’ biocompatibility [BZ_U01; BZ_U04; BZ_U17]; U5 – is able to propose the way of biomaterials’ modification for different branches of regenerative medicine [BZ_U01; BZ_U04].

Competences: K1 – is responsible for his own cells’ cultures [BZ_K04]; K2– is responsible for tasks, that are realized in groups during the tutorial [BZ_K01; BZ_K02 ;BZ_K03]; K3 – is conscious of importance of working in compliance with safety and health rules and keeps rudiments of aseptic working [BZ_K04].

Prerequisites: Histology and cell biology

Course content: Cell culture. Tissue culture. Synthesis of alginate materials. Design of fibrin implants. Suspension of stem cells in fibrin. Suspension of stem cells onto modified metallic biomaterials. Aseptic working in a laminar flow hood. Centrifuge operation. Inverted microscope operation. Cytotoxicity assays (XTT or MTT).

Recommended literature: 1. Stokłosowa S. : Hodowla komórek i tkanek, PWN, Warszawa 2011; 2. Kawiak J. : Seminaria z cytofizjologii dla studentów medycyny weterynarii i biologii, Urban & Partner, Warszawa 2009; 3. Alberts B.: Podstawy biologii komórki T1.-T2. PWN, Warszawa 2009; 4. Mather J.P. Introduction to Cell and Tissue Culture.Theory and Technique, Springer 1998.

Assessment methods:

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