ECTS
ECTS Course Catalogue

Course details
Course code: BBS20183o16
Semester: 2016/2017 winter
Name: Biotechniques in Animal Reproduction
Major: Biology
Study Type: second cycle
Course type: compulsory
Study Semester: 3
ECTS points: 3
Hours (Lectures / Tutorials / Other): 10 / 20 / 0
Lecturer: dr hab. inż. Wojciech Kruszyński; prof. dr hab. Ewa Łukaszewicz, dr Przemysław Pokorny
Language of instruction: Polish / English
The course taught in English if the group has ≥6 students. The course taught in Polish with a possibility of support in English if the group has <6 students. Contact person: dr hab. Wojciech Kruszyński

Learning outcomes: Knowledge: student has the knowledge allowing to interpret the natural phenomena and processes in research work and practical activities; student knows the research methodology obligatory in natural sciences; student formulates research hypotheses and solves basic scientific problems; student knows how to create an interesting multimedia presentation; student knows methods of chemical and instrument al analysis, with special attention paid to physico-chemical basis; student has the knowledge of research methods and techniques used in laboratories; student knows the rules of biological material handling; student has the knowledge essential for laboratory equipment operating; student demonstrates advantages and disadvantages of particular analytical techniques; student describes the methods of mammals, birds and fish reproduction control; student has the knowledge in the field of embryos transfer, in vitro fertilization, control and induction of reproduction cycles, obtaining, maturation and culturing of oocytes, aided reproduction; student knows the basis of embryonic engineering. Skills: student effectively and safely uses research apparatus and measurement devices used in biology. Is able to select suitable measurement devices and measure needed values of physiological and morphological features; student uses the methods applied in laboratory analytics: optical, spectroscopic, electroanalytical, separation ones; student is able to assess factors influencing the result of laboratory analysis avoiding pre- and post-analytical mistakes; student interprets the results of laboratory analysis based on standards and reference values; student collects and interprets the data form analysis and formulates conclusions based on them; student assesses animals reproduction capacity; student controls the reproduction of animals and uses reproduction biotechnology (cryopreservation, sex control); student uses techniques of aided birds reproduction in creation of genetic reserves of birds ex situ in vitro and in declining species protection; student conducts semen collection, spermatozoa assessment, and knows methods of females insemination in various mammal species; student is able to use the in vitro methods of embryos obtaining; student knows females artificial insemination methods.

Competences: Personal and social competences: student demonstrates creativity and is able to determine priorities in task realization taking into account team work; student obeys the rules of occupational health and safety in biological laboratories, demonstrating responsibility for an assessment of dangers resulting from research techniques used; student demonstrates the care of creation of safe work conditions.

Prerequisites: anatomy, biochemistry, zoology, animal physiology, biology of animal growth and reproduction

Course content: biological basis of the controlling of the reproduction of mammals, birds and fish, artificial insemination (collecting, analysis and treatment of semen and its introduction to the reproduction tract of a female), transfer of embryos (selection of donors and recipients, rinsing out of embryos, assessment, storage and introduction to the reproductive tract of recipients), extracorporal fertilization, controlling and development of reproduction cycles, controlling of the offspring sex on the level of gametes and embryos, controlling of fish sex, maturation and culture of oocytes, supported fertilization, extracorporal maturation of embryos, embryonic engineering.

Recommended literature: 1. Bielańska-Osuchowska Z.: Embriologia, PWRiL, Warszawa 1993. 2. Bielański A., Tischner M., Biotechnologia rozrodu zwierząt gospodarskich. Universitas, Kraków 1993. 3. Bieniarz K., Epler P. (1991) Rozród ryb. AR Kraków. 4. Brylińska M. [red.] (2000) Ryby słodkowodne Polski. Wydawnictwo Naukowe PWN, Warszawa. 5. Cryopreservation and Freeze-Drying Protocols – Day J.G. and Stacey G.N. (eds.) Human Press, Totowa, New Jersey. 6. Krzymowski T. red. Biologia rozrodu zwierząt t. 2. Fizjologiczna regulacja procesów rozrodczych samicy. Wydawnictwo UMW, Olsztyn 2007. 7. Muir W.M., Aggrey S.E.: Poultry genetics, breeding and biotechnology. CABI Publishing. 8. Wierzchowski L., Biotechnologia zwierząt- PWN, Warszawa 1997.

Assessment methods: Completion of the course: Completion of the lectures and exercises together, based on the average grade obtained from three separate sections (fish, bird and mammal) and student’s activity during classes. Written control tests (20-30 minutes per each section) concern the lectures and exercises. The presence on the exercises is compulsory, the student may have only one unauthorized absence. In the case of excused absences the student is required to pass the relevant part of the material.

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