ECTS
ECTS Course Catalogue
Course details
Course code: BIS20103o15Semester: 2015/2016 summer
Name: Tissue Engineering)
Major: Bioinformatics
Study Type: second cycle
Course type: compulsory
Study Semester: 5
ECTS points: 6
Hours (Lectures / Tutorials / Other): 30 / 30 / 0
Lecturer: dr hab. Krzysztof Marycz
Language of instruction: Polish
The course exclusively in Polish, with a possibility of support in English
Learning outcomes: Knowledge W1 – knows the principles of aseptic work [BI2_W15]; W2 – knows the classification of cell and tissue cultures [BI2_W04_TP]; W3 – knows methods of mesenchymal stem cells isolation and identyfication [BI2_W04_TP; BI2_W05_TP]; W4 – has knowledge how to design biomaterials [BI2_W05_TP]; W5 – is aware how to use techniques of cell and tissue culture in regenerative medicine [BI2_W05_TP] Skills U1– is able to conduct cell passage and maintains cell culture [BI2_U05]; U2 – is able to freeze cells and to defrost cells’ pellets [BI2_U05]; U3 – is able to determine cells’ morphology and cultures’ condition using inverted light microscope [BI2_U05]; U4 – is able to plan an experiment in order to determine biomaterials’ biocompatibility [BI2_U05; BI2_U06]; U5 – is able to propose the way of biomaterials’ modification for different branches of regenerative medicine [BI2_U05; BI2_U06; BI2_U12]. Personal and social competeces (attitudes) K1 – is responsible for his own cells’ cultures BI2_K03]; K2– is responsible for tasks, that are realized in groups during the tutorial [BI2_K02; BI2_K03]; K3 – is conscious of importance of working in compliance with safety and health rules and keeps rudiments of aseptic working [BI2- _K06].
Competences:
Prerequisites: Histology and cell biology.
Course content: Cell culture. Tissue culture. Synthesis of alginate materials. Design of fibrin implants. Suspension of stem cells in fibrin. Suspension of stem cells onto modified metallic biomaterials. Aseptic working in a laminar flow hood. Centrifuge operation. Inverted microscope operation. Cytotoxicity assays (XTT or MTT).
Recommended literature: Literatura 1. Stokłosowa S. : Hodowla komórek i tkanek, PWN, Warszawa 2011; 2. Kawiak J. : Seminaria z cytofizjologii dla studentów medycyny weterynarii i biologii, Urban & Partner, Warszawa 2009; 3. Alberts B.: Podstawy biologii komórki T1.-T2. PWN, Warszawa 2009; 4. Mather J.P. Introduction to Cell and Tissue Culture.Theory and Technique, Springer 1998.
Assessment methods: Tutorial credit: regularly, once at month, evaluation of students’ ability to maintaining cells' cultures, cells' passage and medium changing, checking the skills of: cells’ counting, cells’ identification via their morphology, evaluation of the ability for planning an experiment concerning biomaterials’ biocompatibility. The presence on the tutorial is obligatory, the student may have only one unexcused absence. In case of the absence, student is required to pass the relevant part of the material. Course credit: students are obligated to write test from material presented during lecture. Final grade is average obtained from marks received from exam and tutorial. Learning stock for credit is 60%. Intended exam time: 60 minutes.
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