Courses list

Wersja polska

Course code: NBSS10133o12
Semester: 2012/2013 winter
Name: Genetics Engineering
Major: Biotechnology
Study Type: first cycle
Course type: compulsory
Study Semester: 7
ECTS points: 4
Hours (Lectures / Tutorials / Other): 24 / 36 / 0
Lecturer: dr Anna Dąbrowska
Language of instruction: Polish


Learning outcomes: Knowledge: After completing the course student knows how to plan a gene cloning to chosen plasmid. Is able to choose the appropriate vector for gene cloning and for the experiment type. He knows the DNA transformation methods to bacterial and eukaryotic cells and how to perform the analysis of obtained recombinants. Is familiar with the activities of basic DNA modification enzymes and can use an appropriate one to achieve the aim of the experiment. He is able to describe how to perform the basic genetic engineering techniques in DNA analysis. Abilities: Student can perform the isolation of genomic/plasmid DNA and evaluate the quality and quantity of the preparation. He, by himself, is able to prepare the restriction analysis (theoretical and practical) and do the necessary calculations. Can design primers for PCR and prepare the electrophoresis. Is able to interpret the obtained results, design the ligation reaction with the necessary calculations. Can suggest the expression system for the chosen protein and plan the scheme of it’s purification. Social competences: The student is aware of the possibilities to use cloning techniques in different fields. He sees the advantages and disadvantages of GMO and express his point of view at that topic. The student is able to work in team, cares for the details of conducted experiments, labware and equipment. Is willing to study new topics.

Competences: The completed course provides students with the minimum set of skills and knowledge to work in different types of diagnostic laboratories and companies using recombinant DNA technology.

Prerequisites: chemistry, biochemistry, biophysics, molecular biology, microbiology

Course content: DNA recombination. Basic molecular biology tools-restriction and modification enzymes, their types and application. Vectors - types and application. The DNA transformation methods to different type of cells. DNA recombination techniques, clone analysis, recombinant proteins. DNA “libraries”. PCR and related techniques , mutagenesis. Methods used for gene expression and promoter sequences analysis. Molecular basics of cancer, gene therapy- selected problems. The use of selected DNA analytical methods in diagnostics. The methods of yeast genome analysis. Yeast characteristics (species, significance, usage, genom arrangement). The application of S.cerevisiae and other yeast species for protein secretion. The tools for yeast transformation (vectors, plaspids, yeast promoters, regulatory sequences, selection markers). Yeast systems used for protein/receptor interaction analysis.

Recommended literature: 1. Biochemia Harpera, Murray R.K., Granner D.K., Mayes P.A., Rodwell V.W., PZWL (kolejne wznowienia od roku 2004); 2. Biochemia, Stryer L., PWN, wyd. V, 2005 3. Biochemistry Voet S., Voet G. V., wyd. Villey & Son, kolejne wydania od roku 1990 4. Biologia Molekularna (krótkie wykłady), Turner P.C., McLennan A.G, Bates A.D., White M.R.H., PWN, (kolejne wydania od roku 2004). 5. Terapia genowa, Szala S. Wyd. PWN, Warszawa 2003 6. Przykłady analiz DNA, Słomski R., Wyd. Akademii Rolniczej im. A. Cieszkowskiego w Poznaniu, (kolejne wydania od r. 2004) 7. Genetyka molekularna Węgleński P. PWN, kolejne wyd. od roku 1995 8. Scientific articles published in recent years.

Assessment methods: Lab activities, positive grade of lab classes and tests, report, exam.

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